ISO INTERNATIONAL STANDARD 5765-1 IDF 79-1 First edition 2002-09-01 Dried milk, dried ice-mixes and processed cheese Determination of lactose content Part 1: Enzymatic method utilizing the glucose moiety of the lactose Lait sec, melanges secs pour cremes glacées et fromages fondus - Deétermination de la teneuren lactose Partie 1: Méthode enzymatique par la voie glucose Reference numbers ISO5765-1:2002(E) ISO IDF 79-1:2002(E) @ ISO and IDF 2002 Not for Resale ISO5765-1:2002(E) IDF 79-1:2002(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In downloading this any liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies and IDF national ?ISOandIDF2002 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic ISO copyright office International Dairy Federation Case postale 56. CH-1211 Geneva 20 Diamant Building·BoulevardAugusteReyers80·B-1030Brussels Tel. + 41 22 749 01 11 Tel. + 32 2 733 98 88 Fax + 41 22 749 09 47 Fax + 32 2 733 04 13 E-mail copyright@iso.org E-mail info@fil-idf.org Web www.iso.org Web www.fil-idf.org Printed in Switzerland @ISO and IDF2002-All rights reserved itted without license from IHS Not for Resale ISO5765-1:2002(E) IDF 79-1:2002(E) Contents Foreword. Foreword. Introduction. 1 Scope. 2 Term and definition.. : Principle .. 4 Reagents 5 Apparatus. 6 Sampling . 7 Procedure.. 7.1 Test to check the procedure 7.2 Preparation of test sample... 7.3 Test portion. 7.4 Reagent blank test 7.5 Deproteination.. 7.6 Determination 8 Calculation and expression of results. 8.1 Calculation.. 8.2 Expression of results.. 9 Precision. 9.1 Interlaboratory trial 8 9.2 Repeatability 9.3 Reproducibility 10 Test report. Annex A (normative) Good Laboratory Practice (GLP) rules for the performance of enzymatic analyses .... 10 Bibliography. ili S unde from IHS Not for Resal