ISO INTERNATIONAL STANDARD 11268-1 Second edition 2012-11-01 Soil quality Effects of pollutants on earthworms- Part 1: Determination of acutetoxicity to Eisenia fetida/Eisenia andrei Qualitedusol-Effetsdespolluantsvis-a-visdesversdeterre- Partie 1:Détermination de la toxicite aigue vis-a-vis de Eisenia fetidal Eiseniaandrei Reference number ISO 11268-1:2012(E) LSO CopyihtInternatinal Organization for Standardization @ISO2012 without license from IHS Not for Resale ISO11268-1:2012(E) COPYRIGHTPROTECTEDDOCUMENT ISO2012 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either IsO at the address below or isO's memberbody in the country of therequester. ISO copyright office Casepostale 56·CH-1211Geneva20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland @ ISO 2012 -All rights reserved No reproduction orr networking permitted without license from IHS Not for Resale ISO11268-1:2012(E) Contents Page Foreword ..iv Introduction 1 Scope 2 Normativereferences 3 Terms and definitions. .2 4 Principle 3 5 Reagents and material .3 6 Apparatus 5 7 Procedure 5 7.1 Experimentaldesign 5 7.2 Preparation oftest mixture 6 7.3 Additionof theearthworms. 8 7.4 Testconditionsandmeasurements 8 7.5 Referencesubstance. 8 8 Calculationandexpressionofresults 8 8.1 Calculation ..... 8 8.2 Expressionof results 8 9 Validity of the test.. 9 10 Statistical analysis.. 9 10.1 General 9 10.2 Single-concentrationtests 9 10.3 Multi-concentrationtests.. 10 11 Testreport. 11 AnnexA (informative)Determinationof the acutetoxicityof chemicals (inparticularpesticides)on earthwormsundertropicaltestconditions.. 12 AnnexB(informative)Culturingof EiseniafetidaandEiseniaandrei... .14 AnnexC (informative) Determination of water holding capacity of artificial soil. .15 AnnexD(informative)Background information ontheacuteeffectofboric acid on earthworms.. Bibliography ili cense from IHS Not for Resale