ISO INTERNATIONAL STANDARD 10801 First edition 2010-12-15 Nanotechnologies Generation of metal nanoparticles for inhalation toxicity testing using the evaporation/condensation method Nanotechnologies Génération de nanoparticules de métal pour essais de toxicite par inhalation en utilisant la methode de condensation/evaporation Reference number ISO 10801:2010(E) @ ISO 2010 THS unde license from IHS Not for Resale ISO 10801:2010(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by Iso member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below COPYRIGHTPROTECTEDDOCUMENT @ISO2010 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either isO at the address below or ISo's member body in the country of the requester. ISO copyright office Case postale 56. CH-1211 Geneva 20 Tel. + 4122749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ ISO 2010 - All rights reserved I without license from IHS Not for Resale ISO 10801:2010(E) Contents Page Foreword Introduction 1 Scope 2 Normative references 3 Terms and definitions 4 Principle 4.1 Generation 4.2 Preparation of system. 5 Requirements. 5.1 Capacity and control... 5.2 Nanoparticle properties 5.3 Exposure chamber atmosphere.. 5.4 System operational safety .... 6 6.1 Requirements for particle size distribution and mass concentration .6 6.2 Particle size distribution measurement. 6.2.1 Sampling with DMAS... 6.2.2 Sampling for microscopy .... 6.3 Mass concentration measured by filter sampling. 6.3.1 Filter sampling for aerosol mass concentration... 6.3.2 Frequency of sampling .... Nanoparticlegeneration specifications 7.1 Test particle puritylimpurities. 7.2 Size range.... 7.3 Number concentration . 7.4 Nanoparticle shape .. 7.5 Stability..... 7.6 Animal exposure. 8 Assessment of results .. .8 9 Test report. ..8 Annex A (informative) Example method for evaporation/condensation generation of silver nanoparticles... 9 Bibliography 21 Copyright International Organization for Standardizalon ghts reserved ii se from IHS Not for Resale

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